Brain Transcriptome Study – Expression profile of long intergenic non-coding RNAs in human brain
A long intergenic non-coding RNA (lincRNA) is generally defined as a > 200 nucleotide (nt) length RNA derived from genomic regions which do not overlap with protein-coding genes. Currently the functions of several lincRNAs have been revealed, yet the functions of these lincRNAs are diverse, including the regulation of chromatin structures and gene expression. They are also involved in brain functions such as MALAT1 in synapse formation. The change in lincRNA expression pattern may affect the normal function of the brain through dis-regulation of clinically important genes. The aim of this study is to conduct computational analysis of lincRNA expression in post-mortem human brain associated with neuronal degeneration and psychiatric disorders, including Alzheimer’s Disease, Autism and Schizophrenia (SZ) using RNA sequencing (RNA-Seq) data. The study includes the isolation of RNAs from three brain regions implicated in SZ: a) dorsolateral prefrontal cortex b) anterior cingulate c) hippocampus of SZ affected and unaffected brains, and performing RNA-Seq, as well as analysing publically available Alzheimer’s and Autism RNA-seq data. This is the first study of expression profiling and annotation of lincRNAs in post-mortem human brains associated with Autism, Alzheimer’s Disease and SZ.